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Electron Microscopy

A Solution Trusted by Electron Microscope Manufacturers and Users

electron microscope illustration

What is Electron Microscopy?

Electron Microscopy (EM) is a technique for obtaining high resolution images using a beam of accelerated electrons as a source of illumination. The wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, meaning electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects.  

There are two main types of electron microscope: the transmission EM (TEM) and the scanning EM (SEM). The TEM is used to view thin specimens through which electrons can pass generating a projection image, and the conventional SEM depends on the emission of secondary electrons from the surface of the specimen. 

Why Image-Pro?

Life Sciences

Axon Tracing

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Use semi-automated tracing tools to easily outline 2D cross-sections of axons which can later be auto-extended along the entire length in 3D.

Required for 2D: 2D Automated Analysis Module

Required for 3D:
3D Visualization Module & 3D Measure & Analyze Extension

Chromatin Analysis

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Chromatin analysis in a post-mitotic nucleus. Image courtesy of EMPIAR dataset 10100. 

 

Requires: 2D Automated Analysis Module

Golgi Analysis

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Use Machine Learning pixel classification to identify the nature of cells within a complex EM image.

Requires:
2D Automated Analysis Module

Muscle Analysis

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Analysis of mitochondria in a mouse cardiac muscle. Image courtesy of Cell Image Library Image 39756.

 

Requires: 2D Manual Measurements Module

Root Analysis

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Analyze plant root cell analysis for size and distribution.

 

Requires: 2D Automated Analysis Module

T.gondii analysis

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Analysis of Toxoplasma gondii. Image courtesy of EMPIAR dataset 10327.

 

Requires: 2D Automated Analysis Module & Cell Biology Protocol Collection


Protocol:
Cell Morphology

Materials Science

Composite Material Analysis

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Measure the area of pores and constituents and calculates the area fraction of a composite material.

Requires: 2D Automated Analysis Module & Materials Protocol Collection

Protocol: Composite Materials

Fiber Thickness​ Analysis

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Measure both the thickness and orientation of fibers using optical or backscatter/secondary electron microscopy images.

Requires: 2D Automated Analysis Module & Materials Protocol Collection

Protocol: Fiber Thickness

Image Stitching: Tiling & Normalization​

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Stitch images together from large scans to provide a larger field of view and a two-pass correction for uneven shading – which may then be analyzed.

Requires:  Image-Pro base product

Layer Thickness: Concentric Analysis

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Measure the thickness of single or multiple concentric material coatings or layers.

Requires: 2D Automated Analysis Module & Materials Protocol Collection

Protocol: Layers Concentric

Particle Count & Size Analysis​

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Measure and classify particles.

 

Requires: 2D Automated Analysis Module & Materials Protocol Collection

 

Protocol: Particles

Phase Analysis

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Measure phases and calculate the area fraction.

 

Requires: 2D Automated Analysis Module & Materials Protocol Collection

 

Protocol: Phase

3D

Axon Tracing

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Automatically generate isosurfaces from a collection(s) of 2D semi-automated outlines. Measure volume, surface, intensity, and unique morphological object measurements.

Requires: 3D Visualization Module & 3D Analysis Extension

3D-EBSD (Ni) Visualization

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Visualize EBSD data and the differences in crystalline structure and crystallographic orientation of the grain.

Requires: 3D Visualization Module

Image Stack Alignment​

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Use automated Fourier or intensity image stack alignment methods to simplify the alignment process of FIB-SEM image stacks.

Requires: 3D Visualization Module & 3D Analysis Extension

NAND Memory

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Visualize stacks of FIB-SEM images and unveil internal details using different slicer options. 

Requires: 3D Visualization Module

Neuron Analysis

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Do more than visual Neurons with 3D Segmentation and Guide Segmentation options. 

Requires: 3D Visualization Module & 3D Analysis Extension

Volume Analysis​

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View, Render, and measure volume data of key elements within your sets. Peel away extra material and spotlight only what is important.

Requires: 3D Visualization Module & 3D Analysis Extension

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