Electron Microscopy
A Solution Trusted by Electron Microscope Manufacturers and Users
What is Electron Microscopy?
Electron Microscopy (EM) is a technique for obtaining high resolution images using a beam of accelerated electrons as a source of illumination. The wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, meaning electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects.
There are two main types of electron microscope: the transmission EM (TEM) and the scanning EM (SEM). The TEM is used to view thin specimens through which electrons can pass generating a projection image, and the conventional SEM depends on the emission of secondary electrons from the surface of the specimen.
Why Image-Pro?
Support 2D images generated by high-res field emission and variable pressure microscopes.
Support 3D images generated by Ion and High kV Transmission microscopes.
Enable an end-to-end solution for all EM customers.
Work with images from nearly all major EM manufacturers.
Provide integrated connection with newer Hitachi systems.
Life Sciences
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Use semi-automated tracing tools to easily outline 2D cross-sections of axons which can later be auto-extended along the entire length in 3D.
Required for 2D: 2D Automated Analysis Module
Required for 3D: 3D Visualization Module & 3D Measure & Analyze Extension
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Chromatin analysis in a post-mitotic nucleus. Image courtesy of EMPIAR dataset 10100.
Requires: 2D Automated Analysis Module
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Use Machine Learning pixel classification to identify the nature of cells within a complex EM image.
Requires: 2D Automated Analysis Module
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Analysis of mitochondria in a mouse cardiac muscle. Image courtesy of Cell Image Library Image 39756.
Requires: 2D Manual Measurements Module
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Analyze plant root cell analysis for size and distribution.
Requires: 2D Automated Analysis Module
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Analysis of Toxoplasma gondii. Image courtesy of EMPIAR dataset 10327.
Requires: 2D Automated Analysis Module & Cell Biology Protocol Collection
Protocol: Cell Morphology
Materials Science
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Measure the area of pores and constituents and calculates the area fraction of a composite material.
Requires: 2D Automated Analysis Module & Materials Protocol Collection
Protocol: Composite Materials
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Measure both the thickness and orientation of fibers using optical or backscatter/secondary electron microscopy images.
Requires: 2D Automated Analysis Module & Materials Protocol Collection
Protocol: Fiber Thickness
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Stitch images together from large scans to provide a larger field of view and a two-pass correction for uneven shading – which may then be analyzed.
Requires: Image-Pro base product
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Measure the thickness of single or multiple concentric material coatings or layers.
Requires: 2D Automated Analysis Module & Materials Protocol Collection
Protocol: Layers Concentric
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Measure and classify particles.
Requires: 2D Automated Analysis Module & Materials Protocol Collection
Protocol: Particles
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Measure phases and calculate the area fraction.
Requires: 2D Automated Analysis Module & Materials Protocol Collection
Protocol: Phase
3D
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Automatically generate isosurfaces from a collection(s) of 2D semi-automated outlines. Measure volume, surface, intensity, and unique morphological object measurements.
Requires: 3D Visualization Module & 3D Analysis Extension
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Visualize EBSD data and the differences in crystalline structure and crystallographic orientation of the grain.
Requires: 3D Visualization Module
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Use automated Fourier or intensity image stack alignment methods to simplify the alignment process of FIB-SEM image stacks.
Requires: 3D Visualization Module & 3D Analysis Extension
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Visualize stacks of FIB-SEM images and unveil internal details using different slicer options.
Requires: 3D Visualization Module
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Do more than visual Neurons with 3D Segmentation and Guide Segmentation options.
Requires: 3D Visualization Module & 3D Analysis Extension
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View, Render, and measure volume data of key elements within your sets. Peel away extra material and spotlight only what is important.
Requires: 3D Visualization Module & 3D Analysis Extension
Image-Pro Has Tools for Your Acquisition Type.
Get Started with Image-Pro for Electron Microscopy
