
Light Microscopy
A Solution Trusted by Light Microscope
Manufacturers and Users

What is Light Microscopy?
Light Microscopy is a technique for generating magnified images of small objects using visible light and a system of lenses. There are a variety of types including simple and compound lens, stereo, inverted, polarizing, phase contrast, Epifluorescence, Confocal, 2-Photon, and more.
A wide range of illumination techniques are used to generate improved contrast and, in some cases, the addition of standard fluorescent dyes to the sample improve localization of structures and inform about relationships.
Why Image-Pro?
Supercharge your manual microscopes through powerful capture tools that improve collaboration, handles complex datasets and performs high-volume image analysis. It also optimizes data with filters, coloring, alignment and more all the while saving time on repetitive tasks.Â
Supercharge manual microscopes by adding powerful capture tools.
Improve collaboration with support for OME.TIF and all major microscope formats.
Handles complex multi-dimensional datasets with ease.
Optimize your light microscope data with filters, coloring, alignment, and more.
Perform expert, high-volume image analysis on nearly any image.
Save time on repetitive tasks using custom macros and a customizable user interface.
Brightfield & Stereo

Component Inspection & Measurement
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Supported by a wide range of manual measurement and automated measurement features, Image-Pro can quickly be set up to analyze component inspection and measurement.
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Requires: 2D Measurement Module and/or 2D Automated Analysis Module

Confluence
Analysis
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Requires: 2D Automated Analysis Module & Cell Biology Protocol Collection
Protocol: Confluence

Extended
Depth of Field (EDF) of Large Samples
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Capture beautifully focused images, regardless of depth, with Live EDF. No automated z motor is required.
Requires: 2D Capture Module

Object Count &
Size Analysis​
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Count and classify objects in the region of interest.Â
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Requires: 2D Automated Analysis Module & Essentials Protocol Collection (included)
Protocol: Object Count

Paint Color
Quality Assurance
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Perform absolute color measurements or color differences in CIE L*a*b*® or XYZ color coordinates. It’s also just as easy to color correct from a reference image.
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Requires: 2D Measurements Module

Tissue Stain
Analysis
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Using machine learning-based Smart Segmentation, Image-Pro may be quickly trained to identify stained tissue areas in a reliable and repeatable manner.
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Requires: 2D Automated Analysis Module & Essentials Protocol Collection (included)
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Protocol: Percent Area
Fluorescence

3D Reconstruction of Multi-Channel Stacks
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Reconstruct 3D stacks across multiple fluorescent channels for visualization of structure and analysis of 3D morphology and colocalization.
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Requires: 3D Visualization Module &Â 3D Measure & Analyze Extension

Angiogenesis Analysis
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Measure the growth and diameter of fluorescently labeled blood vessels.
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Requires: 2D Automated Analysis Module & Cell Biology Plus Protocol Collection
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Protocol: Angiogenesis

Apoptosis
Analysis
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Measure apoptosis based on caspase activation of fluorescently labeled cells.
Requires: 2D Automated Analysis Module & Cell Biology Protocol Collection
Protocol: Apoptosis

Capture Multi-Channel Image Sets
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Expand the capabilities of any microscope to capture multiple channels into an image set.
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Requires: 2D Capture Module & Multi-Channel Capture Extension

Real-Time or
Post-Acquisition Deconvolution
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Elevate your capture system to now deliver crisp, high fidelity, fluorescent images during preview and capture.
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Required for Live: 2D Capture Module & Real-Time Deconvolution Extension
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Required for Post-Acquisition: AutoQuant Deconvolution Module

Translocation
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Measure either the total cellular area of transport of a fluorescently labeled target protein between fluorescently labeled nuclear and cytoplasmic compartments of just the area above threshold.
Requires: 2D Automated Analysis Module & Cell Biology Plus Protocol Collection
Protocol: Translocation
Image-Pro Has Tools for Your Acquisition Type.
Get Started with Image-Pro for Light Microscopy