Live/Dead for Cell Viability
A cell viability assay measures the proportion of live, healthy cultured cells, commonly used to evaluate cell responses to drugs or chemical agents (Kamiloglu et al., 2020). Viability is assessed by staining live cells (e.g., Calcein-AM, which fluoresces in living cells), dead cells (e.g., Propidium Iodide, which stains DNA in dying or dead cells), or both simultaneously.
The Image-Pro Live/Dead for Cell Viability protocol is compatible with combinations of a) live and total cells, b) dead and total cells, or c) live, dead and total cells. Pre-configured versions of the protocol can be loaded for any of these combinations. The protocol allows the analysis of large volumes of data in complex formats such as multi-well plates with little to no image analysis experience.
Techniques: Fluorescence
How it works
Select Channel
Select the channels that contain total cells, labeled live cells, and labeled dead cells.
Find Cells or Nuclei
Find cells or nuclei with a pre-trained deep learning model, machine learning, or threshold segmentation.
Find Live/Dead Cells
Identify cells that are labeled as ‘live’ or 'dead' with either threshold segmentation or machine learning segmentation.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Live Cell Count
- • Dead Cell Count
- • Total Cells
- • Percentage Live
- • Percentage Dead
- • Custom user defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Protocol Collection
Live/Dead Cell Viability Protocol
AI Deep Learning
Life Science Models
Fluorescent Cells Model
Recommended Package
Literature spotlight
- Parsons, D., Meredith, K., Rowlands, V. J., Short, D., Metcalf, D. G., & Bowler, P. G. (2016). Enhanced performance and mode of action of a novel antibiofilm Hydrofiber® wound dressing. BioMed Research International, 2016(1), 7616471.
- Kesler Shvero, D., Zaltsman, N., Weiss, E. I., Polak, D., Hazan, R., & Beyth, N. (2016). Lethal bacterial trap: cationic surface for endodontic sealing. Journal of Biomedical Materials Research Part A, 104(2), 427-434.
- Xavier, M. V., Macedo, M. F., Benatti, A. C. B., Jardini, A. L., Rodrigues, A. A., Lopes, M. S., ... & Kharmandayan, P. (2016). PLLA synthesis and nanofibers production: viability by human mesenchymal stem cell from adipose tissue. Procedia CIRP, 49, 213-221.4
