Cell Apoptosis
Apoptosis, or programmed cell death, is a vital process observed in metazoans and some single-celled eukaryotes like yeast. It plays a crucial role in cell turnover, immune responses, development, hormone-dependent atrophy, and chemical-induced cell death (Lawen, 2003). A failure to initiate apoptosis in mutated cells can lead to cancer, making its analysis essential in cancer research.
Morphological changes during apoptosis include cell separation, membrane disintegration, and blebbing (Wyllie, 1997). Caspase proteins, critical mediators of apoptosis, are commonly used in assays to identify apoptotic cells.
The Image-Pro Apoptosis protocol enables efficient and accurate analysis of apoptosis, even for users with limited image analysis expertise. Its user-friendly design simplifies large-scale data processing, saving time and ensuring reproducibility in research.
Techniques: Fluorescence
How it works
Select Channel
Select the channel that contains labeled nuclei and the channel that contains labeled apoptosis marker.
Find Nuclei
Find nuclei with a pre-trained deep learning model, machine learning, or threshold segmentation.
Segment Marker
Find the apoptosis marker with a pre-trained deep learning model, machine learning, or threshold segmentation.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Total Cell Count
- • Total Apoptotic Cell Count
- • Apoptotic Cell Percentage
- • Custom user-defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Protocol Collection
Cell Apoptosis Protocol
AI Deep Learning
Life Science Models
Fluorescent Cells Model
Recommended Package
Literature spotlight
- Li, Y., Chen, X., Li, P., Xiao, Q., Hou, D. and Kong, X., 2020. CD47 antibody suppresses isoproterenol-induced cardiac hypertrophy through activation of autophagy. American Journal of Translational Research, 12(9), p.5908.
- Sun, Q., Song, R., Yang, L., Wan, Q., Shuai, Y., Wang, X. and Xu, J., 2024. BMP7 attenuates propofol induced neuron apoptosis via modulating JNK and AKT signaling.
- Xu, Z., Li, Y., Pi, P., Yi, Y., Tang, H., Zhang, Z., Xiong, H., Lei, B., Shi, Y., Li, J. and Sun, Z., 2024. B. glomerulata Promotes Neuroprotection Against Ischemic Stroke by Inhibiting Apoptosis through the Activation of PI3K/AKT/mTOR Pathway. Phytomedicine, p.155817.
