Angiogenesis (Tube Formation)
Angiogenesis—the formation and remodeling of new blood vessels—is a critical process in various research fields, including oncology, developmental biology, wound healing, dermatology, vascular disease, hematology, and ophthalmology (Ucuzian and Greisler, 2007). Experimental models using cultured endothelial cells are widely employed to study the effects of agonists on angiogenesis. These models typically involve seeding endothelial cells onto culture dishes coated with adhesive proteins or gels composed of collagen, fibrin, or Matrigel (Vailhé et al., 2001).
The Image-Pro Angiogenesis protocol streamlines the analysis of these models, enabling researchers to process large datasets automatically with minimal image analysis expertise. Powered by a pre-trained deep learning model, it quickly and accurately detects and quantifies blood vessels, saving time and ensuring reproducible results.
Techniques: Brightfield, Fluorescence
How it works
Select Channel
Select the channel that contains vessels.
Find Cells
Use simple sliders to find colonies of progenitor cells.
Find Tubes
Find tubes using either simple sliders or a pre-trained deep learning model.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Total Tube Length
- • Average Tube Length
- • Max Tube Lengths
- • Branch Count
- • Tube Count
- • Snapshots of analyzed fields
- • Custom user-defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Plus Protocol Collection
Angiogenesis Protocol
AI Deep Learning
Life Science Models
Fluorescent Dendrites Model
Recommended Package
Literature spotlight
- Li, Z., Fan, H., Cao, J., Sun, G., Wang, S., Lv, J., Xuan, Z., Xia, Y., Wang, L., Zhang, D. and Xu, H., 2021. Natriuretic peptide receptor a promotes gastric malignancy through angiogenesis process. Cell Death & Disease, 12(11), p.968.
- Li, B., Zhang, Y., Yin, R., Zhong, W., Chen, R. and Yan, J., 2020. Activating CD137 signaling promotes sprouting angiogenesis via increased VEGFA secretion and the VEGFR2/Akt/eNOS pathway. Mediators of inflammation, 2020(1), p.1649453.
- Suleiman, L., Muataz, Y., Négrier, C. and Boukerche, H., 2021. Protein S-mediated signal transduction pathway regulates lung cancer cell proliferation, migration and angiogenesis. Hematology/Oncology and Stem Cell Therapy.
