Angiogenesis (Tube Formation)
Angiogenesis is the formation and remodeling of new blood vessels. Experimental cultured cell-based models to test the effects of agonists on this process are an essential element of numerous research areas including oncology, developmental biology, wound healing, dermatology, peripheral and coronary vascular disease, hematology and ophthalmology (Ucuzian and Greisler 2007). Such assays generally involve the seeding of endothelial cells onto plastic culture dishes that have been coated with adhesive proteins or a gel made of either collagen, fibrin, or Matrigel (Vailhé et a. 2001).
The Image-Pro Angiogenesis protocol makes the analysis of your experimental models easy. You can analyze large datasets automatically with little to no image analysis experience while finding vessels with a pre-trained deep learning model.
Techniques: Fluorescence
How it works
Select Channel
Select the channel that contains vessels.
Find Cells
Use simple sliders to find colonies of progenitor cells.
Find Tubes
Find tubes using either simple sliders or a pre-trained deep learning model.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Total Tube Length
- • Average Tube Length
- • Max Tube Lengths
- • Branch Count
- • Tube Count
- • Snapshots of analyzed fields
- • Custom user defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Plus Protocol Collection
Angiogenesis Protocol
AI Deep Learning
Life Science Models
Fluorescent Dendrites Model
Recommended Package
Literature spotlight
- Li, Z., Fan, H., Cao, J., Sun, G., Wang, S., Lv, J., Xuan, Z., Xia, Y., Wang, L., Zhang, D. and Xu, H., 2021. Natriuretic peptide receptor a promotes gastric malignancy through angiogenesis process. Cell Death & Disease, 12(11), p.968.
- Li, B., Zhang, Y., Yin, R., Zhong, W., Chen, R. and Yan, J., 2020. Activating CD137 signaling promotes sprouting angiogenesis via increased VEGFA secretion and the VEGFR2/Akt/eNOS pathway. Mediators of inflammation, 2020(1), p.1649453.
- Suleiman, L., Muataz, Y., Négrier, C. and Boukerche, H., 2021. Protein S-mediated signal transduction pathway regulates lung cancer cell proliferation, migration and angiogenesis. Hematology/Oncology and Stem Cell Therapy.
