Cell Apoptosis
Apoptosis is programmed cell death seen in metazoans and some single-celled eukaryotes like yeast. It plays a vital role in processes such as cell turnover, immune response, development, and hormone-dependent atrophy, and in chemical-induced cell death (Lawen 2003).
The failure to initiate apoptosis in mutated cells contributes to cancer, making its analysis crucial in cancer research. Morphological changes during apoptosis include cell separation, membrane structure loss, and blebbing (Wyllie 1997). Caspase proteins are key to apoptosis and are used in assays to detect apoptotic cells.
Image-Pro Apoptosis protocol simplifies large-scale analysis of these cells, even for users with minimal image analysis experience.
Techniques: Fluorescence
How it works
Select Channel
Select the channel that contains labeled nuclei and the channel that contains labeled apoptosis marker.
Find Nuclei
Find nuclei with a Pre-Trained Deep Learning Model, Machine Learning, or Threshold Segmentation.
Segment Marker
Find the apoptosis marker with a Pre-Trained Deep Learning Model, Machine Learning, or Threshold Segmentation.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Total Cell Count
- • Total Apoptotic Cell Count
- • Apoptotic Cell Percentage
- • Custom user defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Protocol Collection
Cell Apoptosis Protocol
AI Deep Learning
Life Science Models
Fluorescent Cells Model
Recommended Package
Literature spotlight
- Li, Y., Chen, X., Li, P., Xiao, Q., Hou, D. and Kong, X., 2020. CD47 antibody suppresses isoproterenol-induced cardiac hypertrophy through activation of autophagy. American Journal of Translational Research, 12(9), p.5908.
- Sun, Q., Song, R., Yang, L., Wan, Q., Shuai, Y., Wang, X. and Xu, J., 2024. BMP7 attenuates propofol induced neuron apoptosis via modulating JNK and AKT signaling.
- Xu, Z., Li, Y., Pi, P., Yi, Y., Tang, H., Zhang, Z., Xiong, H., Lei, B., Shi, Y., Li, J. and Sun, Z., 2024. B. glomerulata Promotes Neuroprotection Against Ischemic Stroke by Inhibiting Apoptosis through the Activation of PI3K/AKT/mTOR Pathway. Phytomedicine, p.155817.
