Cell Autophagy
Autophagy is a conserved cellular recycling process in eukaryotes that removes unnecessary or damaged components via a lysosome-dependent mechanism. It plays a crucial role in cell survival and maintenance and dysfunction in autophagy is linked to various human diseases (Parzych et al. 2014).
Assays using fluorescent dyes to label autophagosomes are widely available (Ding and Hong 2020) and can be efficiently analyzed with the Image-Pro Autophagy Protocol, enabling large-scale data analysis in complex formats like multi-well plates, even with minimal image analysis experience.
Techniques: Fluorescence
How it works
Select Channel
Select the channel that contains labeled cells (this can be a cellular or nuclear dye) and the channel that contains labeled autophagosomes.
Find Cells
Find cells or nuclei with either a Pre-Trained Deep Learning Model, Machine Learning, or Threshold Segmentation.
Find Autophagosomes
Find autophagosomes with either a Pre-Trained Deep Learning Model, Machine Learning, or Threshold Segmentation.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Autophagosomes per cell
- • Mean autophagosome diameter
- • Mean autophagosome intensity
- • Mean autophagosome area
- • Autophagosome count
- • Custom user defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Plus Protocol Collection
Cell Autophagy Procol
AI Deep Learning
Life Science Models
Fluorescent Nuclei Model
Recommended Package
Literature spotlight
- Ji, L., Wang, Z.H., Zhang, Y.H., Zhou, Y., Tang, D.S., Yan, C.S., Ma, J.M., Fang, K., Gao, L., Ren, N.S. and Cheng, L., 2022. ATG7-enhanced impaired autophagy exacerbates acute pancreatitis by promoting regulated necrosis via the miR-30b-5p/CAMKII pathway. Cell Death & Disease, 13(3), p.211.
- Wu, Dongle, Xuan Sun, Yiwei Zhao, Yuanbo Liu, Ziqi Gan, Zhen Zhang, Xin Chen, and Yang Cao. "Strontium ranelate inhibits osteoclastogenesis through NF-κB-pathway-dependent autophagy." Bioengineering 10, no. 3 (2023): 365.
- Zou, Y., Zhang, X., Chen, X.Y., Ma, X.F., Feng, X.Y., Sun, Y., Ma, T., Ma, Q.H., Zhao, X.D. and Xu, D.E., (2024) Contactin-Associated protein1 Regulates Autophagy by Modulating the PI3K/AKT/mTOR Signaling Pathway and ATG4B Levels in Vitro and in Vivo. Molecular Neurobiology, pp.1-17.
