Lipid Droplet
Lipid droplets are essential cytoplasmic organelles that store neutral lipids, playing a crucial role in energy metabolism and lipid homeostasis. These organelles are found in a variety of organisms, including animals, plants, fungi, and bacteria (Welte & Gould, 2017). Lipid droplets are dynamic, and their number, size, and composition can vary significantly within a homogenous cell population as well as across different cell types (Thiam & Beller, 2017).
The study and quantification of lipid droplets are essential for understanding various cellular processes, including metabolic regulation and disease mechanisms. The Image-Pro Lipid Droplets protocol simplifies this analysis, offering tools for the detection, quantification, and characterization of lipid droplets across large datasets. It supports complex formats, such as multi-well plates, and enables users to conduct high-throughput analysis with minimal image analysis experience.
Techniques: Brightfield, Fluorescence
How it works
Select Channel
Select the channel that contains cells and the channel that contains labeled lipid droplets.
Find Cells
Find cells with a pre-trained deep learning model, machine learning, or threshold segmentation.
Find Lipid Droplets
Find lipid droplets with a pre-trained deep learning model, machine learning, or threshold segmentation.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Total Number of Cells
- • Total Number of Cells with Three or More Lipid Droplets
- • Percentage of Cells with Three of More Lipid Droplets
- • Mean Droplets Per Cell
- • Custom user-defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Protocol Collection
Lipid Droplet Protocol
AI Deep Learning
Life Science Models
Fluorescent Cells Model
Recommended Package
Literature spotlight
- Li, L. L., Wang, D., Ge, C. Y., Yu, L., Zhao, J. L., & Ma, H. T. (2018). Dehydroepiandrosterone reduced lipid droplet accumulation via inhibiting cell proliferation and improving mitochondrial function in primary chicken hepatocytes. Physiological Research, 67(3), 443-456.
- Long-Long, L. I., Dian, W. A. N. G., Chong-Yang, G. E., Lei, Y. U., Jin-Long, Z. H. A. O., & Hai-Tian, M. A. (2018). Dehydroepiandrosterone reduced lipid droplet accumulation via inhibiting cell proliferation and improving mitochondrial function in primary chicken 2 hepatocytes 3. Physiological Research, 67(3).
- Yang, J., Ren, J., Song, J., Liu, F., Wu, C., Wang, X., ... & Chen, L. (2013). Glucagon-like peptide 1 regulates adipogenesis in 3T3-L1 preadipocytes. International journal of molecular medicine, 31(6), 1429-1435.
