Lipid Droplet
Lipid droplets are cytoplasmic organelles that store neutral lipids and are critically important for energy metabolism. They are ubiquitous organelles, present in animals, plants, fungi, and even bacteria (Welte & Gould 2017).
The number, size and composition of lipid droplets vary greatly within cells in a homogenous population as well as in different cell types (Thiam & Beller 2017).
The study and quantification of these important organelles is made easy by the Image-Pro Lipid Droplets protocol, which allows analysis of large volumes of data in complex formats such as multi-well plates with little to no image analysis experience.
Techniques: Fluorescence
How it works
Select Channel
Select the channel that contains cells and the channel that contains labeled lipid droplets.
Find Cells
Find cells with a pre-trained deep learning model, machine learning, or threshold segmentation.
Find Lipid Droplets
Find lipid droplets with a pre-trained deep learning model, machine learning, or threshold segmentation.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Total Number of Cells
- • Total Number of Cells with Three or More Lipid Droplets
- • Percentage of Cells with Three of More Lipid Droplets
- • Mean Droplets per Cell
- • Custom user defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Protocol Collection
Lipid Droplet Protocol
AI Deep Learning
Life Science Models
Fluorescent Cells Model
Recommended Package
Literature spotlight
- Li, L. L., Wang, D., Ge, C. Y., Yu, L., Zhao, J. L., & Ma, H. T. (2018). Dehydroepiandrosterone reduced lipid droplet accumulation via inhibiting cell proliferation and improving mitochondrial function in primary chicken hepatocytes. Physiological Research, 67(3), 443-456.
- Long-Long, L. I., Dian, W. A. N. G., Chong-Yang, G. E., Lei, Y. U., Jin-Long, Z. H. A. O., & Hai-Tian, M. A. (2018). Dehydroepiandrosterone reduced lipid droplet accumulation via inhibiting cell proliferation and improving mitochondrial function in primary chicken 2 hepatocytes 3. Physiological Research, 67(3).
- Yang, J., Ren, J., Song, J., Liu, F., Wu, C., Wang, X., ... & Chen, L. (2013). Glucagon-like peptide 1 regulates adipogenesis in 3T3-L1 preadipocytes. International journal of molecular medicine, 31(6), 1429-1435.
