Nuclei & Mitochondria (TEM)
Nuclei, the defining feature of eukaryotic cells, serve as the cellular compartment housing chromosomes. Mitochondria, often referred to as the powerhouses of eukaryotic cells, are equally fundamental. These double membrane-bound organelles are familiar to all cell biologists from textbook diagrams and electron micrographs. However, they remain notoriously challenging to automatically segment in electron micrographs, where numerous organelles exhibit similar staining characteristics and display highly variable shapes and sizes.
Traditionally, studying organelle ultrastructure relied on slow, labor-intensive manual segmentation (Perez et al., 2014). The Image-Pro pre-trained Nuclei TEM and Mitochondria TEM deep learning models solve this problem. Used alone or with the Nuclei and Mitochondria (EM) protocol, these tools enable fast, accurate TEM data analysis—even for users with little to no image analysis experience.
Techniques: FIB-SEM, TEM
How it works
Select Your Image
Select your TEM image and open the Nucleus and Mitochondria (EM) protocol.
Find Nuclei
Find nuclei with the TEM Nuclei pre-trained deep learning model.
Find Mitochondria
Find mitochondria with the TEM Mitochondria pre-trained deep learning model.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Nucleus Count
- • Mitochondria Count
- • Mean Nuclear Area
- • Mean Mitochondria Area
- • Mean Nuclear Aspect Ratio
- • Mean Mitochondria Aspect Ratio
- • Mean Nuclear Circularity
- • Mean Mitochondria Circularity
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology EM Protocol Collection
Nuclei and Mitochondria (EM) Protocol
AI Deep Learning
Life Science Models
TEM Nuclei Model
TEM Mitochondria Model
Recommended Package
