Cell Confluence
Confluence refers to the extent to which cultured adherent cells occupy the surface of a 2D substrate, such as a cell culture vessel. It is measured as the percentage of the surface area covered by cells. For example, 50% confluence means half of the available surface is covered, while 100% confluence indicates complete coverage with no remaining space for monolayer growth.
Precise and reliable measurement of cell confluence is critical for determining the optimal time to split and passage cell cultures or to harvest cells for experimental treatments. The Image-Pro Cell Confluence protocol ensures reproducible measurements, enabling high-quality, reliable data essential for successful cell culture experiments.
Techniques: Brightfield, Fluorescence
How it works
Select Channel
Select the channel that contains unlabeled cells.
Set Object Diameter
Set the diameter of unlabeled cells.
Find Cells
Measure the area occupied by cells with either a pre-trained deep learning model or machine learning.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Confluence Percentage
- • Cell Count
- • Custom user-defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Protocol Collection
Cell Confluence Protocol
AI Deep Learning
Life Science Models
Label Free Cells Model
Recommended Package
