Cell Confluence

Confluence is the degree to which cultured adherent cells occupy the space available on a 2D substrate such as the surface of a cell culture vessel. Confluence is measured as the percentage area occupied by cells. A confluence of 50% indicates that half of the available surface area is occupied by cells, while confluence of 100% indicates that all the space is occupied by cells and there is no further room for cells to grow as a monolayer.

Accurate and reliable measurement of Cell Confluence is essential in determining the correct time to split and passage cell cultures, as well as determining the correct time to harvest cells for experimental treatments. Accurate and reproducible measurements are vital for generating high-quality, reliable data (Nare 2022).