Cytoplasm-Nucleus
Translocation
Eukaryotic cells feature a double membrane-bound nucleus, the Nuclear Envelope (NE), which separates genetic material from the cytoplasm. The NE consists of inner and outer membranes (Matsuda & Mofrad, 2022) and is perforated by nuclear pore complexes (NPCs). These NPCs enable the active transport of proteins and other molecules between the nucleus and cytoplasm, regulating essential cellular processes (Görlich, 1998; Tijana & Anton, 2017).
The distribution of proteins and macromolecules between these compartments is crucial for understanding cell cycle stages, disease states, and cell functions. The Image-Pro Translocation protocol simplifies this analysis by providing a nuclear-to-cytoplasmic fluorescence ratio and other compartment-specific intensity measurements. Compatible with pre-trained deep learning models, it facilitates high-throughput analysis of complex data formats, such as multi-well plates, with little to no image analysis expertise.
Techniques: Fluorescence
How it works
Select Channel
Select the channels that contain labeled nuclei, cells, and the translocation target.
Find Cells & Nuclei
Find cells and nuclei with a pre-trained deep learning model, machine learning, or threshold segmentation.
Separate from Background
Separate the translocation target from the background with either threshold segmentation or machine learning.
Quantitative results
Automatically generate tables, heat maps, charts and even complex bespoke reports.
Measurement parameters supported
- • Cytoplasmic Intensity
- • Nuclear Intensity
- • Nuclear/Cytoplasmic difference in intensity
- • Nuclear/Cytoplasmic Intensity Ration
- • Custom user defined measurements
Solution requirements
Required Modules
Base
2D Automated Analysis
Cell Biology Protocol Collection
Translocation Protocol
AI Deep Learning
Life Science Models
Fluorescent Nuclei Model
Recommended Package
Literature spotlight
- Hu, W., Chen, M., Wang, W., Huang, F., Tian, X., & Xie, L. (2022). Pomelo Peel Essential Oil Ameliorates Cerebral Ischemia‐Reperfusion Injury through Regulating Redox Homeostasis in Rats and SH‐SY5Y Cells. Oxidative medicine and cellular longevity, 2022(1), 8279851.
- Xia, Q., Mao, M., Zeng, Z., Luo, Z., Zhao, Y., Shi, J., & Li, X. (2021). Inhibition of SENP6 restrains cerebral ischemia-reperfusion injury by regulating Annexin-A1 nuclear translocation-associated neuronal apoptosis. Theranostics, 11(15), 7450.
- Sebastian, J. A., Moore, M. J., Berndl, E. S., & Kolios, M. C. (2021). An image-based flow cytometric approach to the assessment of the nucleus-to-cytoplasm ratio. PLoS One, 16(6), e0253439.
